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lihlahisoa

Magnetic Bead DNA Extraction Kit Rapid Efficient Efficient DNA Purification Collection Kit

Tlhaloso e Khutšoanyane:

CY-F006-10 (50preps cell)

CYF006-11 (100preps-cells)

CY-F006-12 (200preps-cell)

CY-F006-20 (50preps-saliva)

CY-F006-21 (100preps-saliva)

CY-F006-22 (200preps-saliva)

Tlhaloso ea ho paka:

Batho ba 50 / lebokose, batho ba 100 / lebokose, batho ba 200 / lebokose


Lintlha tsa Sehlahisoa

Li-tag tsa Sehlahisoa

Selelekela sa Sehlahisoa:

The Iclean DNA Extraction Kit e fana ka mokhoa o potlakileng le o sebetsang oa sefaha sa makenete ho hloekisa le ho ntša DNA (ho kenyeletsoa genomic, mitochondrial le DNA ea kokoana-hloko) ho tloha liseleng tse bolokiloeng, mathe, maro a 'mele, le lisele tsa letlalo, lisele tsa letlalo, lisele tsa baktheria jj.

Mehlala ea baeloji Mehlala e ka bolokoa sebakeng sa rona sa ho boloka nako ho isa ho matsatsi a 30 mochesong oa kamore pele e sebetsoa ntle le tahlehelo e hlokomelehang ea kotulo ea DNA kapa boleng (~ matsatsi a 30 haeba u ka bolokoa maemong a leqhoa)

DNA ea boleng bo holimo ea genomic e ka hloekisoa ka metsotso e 15 ntle le ho ntšoa ha phenol/chloroform kapa pula ea tahi, ka poelo e tloaelehileng ea DNA ea 8 μg ka buccal swab.DNA e hloekisitsoeng, e ka bang 20-30 kb, e loketse lits'ebetso tse tlase tse kang PCR kapa liketso tse ling tsa enzymatic.

Melemo ea Sehlahisoa

Ts'ebetso e phahameng, ho ntšoa ha DNA ka mokhoa o le mong, ho tlosa protheine e sa hloekang le metsoako e meng ea lintho tse phelang liseleng.Likhechana tsa DNA tse nkiloeng li kholo, li hloekile, li tsitsitse ebile li ka tšeptjoa ka boleng.

1. Theknoloji e thehiloeng ho lifaha tsa Magnetic ho arola DNA ea genomic ntle le tlhoko ea lik'hemik'hale tse kotsi, centrifugation, kapa vacuum manifolds, phenol le ethanol precipitation.

2.Ho hloekisa ka potlako le ka mokhoa o nepahetseng ha DNA ea genomic ho tloha ho batho ba buccal swabs ka nako e ka tlase ho metsotso ea 15 ka mor'a ho lokisetsa sampole le lysis.

3.Simple lysis le Proteinase K ntle le tlhokahalo ea lysis efe kapa efe ea mechine.

4.Tšilafatso e fokolang le RNA.

5.The DNA e hloekisitsoeng ea genomic e bonts'a ts'ebetso e ntlafetseng ea lits'ebetso tse kenyeletsang PCR.

6.E kenyelletsa kit e etselitsoeng ts'ebetso e ikemetseng ea lisampole tse ngata ka har'a lipoleiti tsa liliba tse 96 tse sebelisang roboto e sebetsanang le metsi.

Tšebeliso ea Sehlahisoa:

Bakeng sa tlhoekiso le ho arola DNA (ho kenyeletsoa genomic, mitochondrial, bacterial, parasite & viral DNA) ho tsoa liseleng, mathe, maro a 'mele, le buccal, cervical, lisele tsa letlalo, lisele tsa baktheria, lisele, li-swabs, CSF, maro a 'mele, lisele tse hlatsoitsoeng tsa moroto. .

Litaelo tsa Sehlahisoa

1. Pele o sebelisa li-reagents tsa nucleic acid

①.Fetisetsa solvent ea proteinase k ka har'a phofo e omisitsoeng e omisitsoeng e nang le proteinase k 'me u kopanye hantle.

②.Eketsa 18ml le 42ml ea ethanol e feletseng ho CY3 le CY4 ea mohlala CY-F006-10 (50preps-cells) le CY-F006-20 (50preps-saliva), 'me u kopanye hantle.

③.Kenya 36ml le 84ml ethanol e feletseng ho CY3 le CY4 ea mohlala CY-F006-11 (100preps-cells) le CY-F006-21 (100preps-saliva) 'me u kopanye hantle.

 

2. Mehato ea ho ntša Swab:

① Pokello e ommeng ea Swab, eketsa mokelikeli oa 0.6ml CY1, 10ul proteinase K, kopanya hantle, o e behe ka har'a incubator ea moea e mocheso oa likhato tse 65 tsa Celsius 'me u e kenye metsotso e 30 (kapa pokello e metsi: mohlala oa centrifuge tube e nang le swab plus preservation solution e centrifuged at 12000 rpm bakeng sa motsotso o le 1, Boloka mocheso, tlosa matla a phahametseng matla.Eketsa mokelikeli oa 0.6ml CY1, 10ul proteinase k, kopanya hantle, o e behe ka har'a incubator ea moea ka likhato tse 65 tsa Celsius 'me u e kenye metsotso e 30).

②.Tlosa swab le centrifuge ho 12000rpm bakeng sa 1min.

③.Ntša supernatant eohle ho tube e ncha ea centrifuge 'me u etse liteko.

④.Eketsa mokelikeli oa 0.25ml CY2, 10ul lifaha tsa makenete * (tse sisinyeha hantle pele li sebelisoa), kopanya hantle bakeng sa 12min, e behe holim'a sethala sa makenete 'me u e tlohelle hore e eme bakeng sa 30s,' me u monye mokelikeli.

⑤ Eketsa 0.6ml ea mokelikeli oa CY3, kopanya hantle bakeng sa 3min, e behe holim'a sethala sa makenete 'me u se tlohele hore se eme 30s ho monya mokelikeli.

⑥.Kenya 0.6ml ea CY4 mokelikeli, kopanya 3min, e behe holim'a sethala sa makenete 'me u e tlohelle e eme bakeng sa 30s, e monye metsi.

⑦.Pheta mehato ②⑥

⑧.E omisa bakeng sa 10-20min mocheso oa kamore, eketsa metsi a 50ul CY5 bakeng sa elution, kopanya hantle, e behe holim'a sethala sa makenete 'me u se tlohele se eme bakeng sa 30s, ebe u fetisetsa mokelikeli ho tube e ncha ea centrifuge.

⑨.Lekanya OD

 

3. Mohato oa ho ntša mathe

① Centrifuge ho 12000rpm bakeng sa 1min e nang le mathe le motsoako oa ho boloka

② Boloka lipula le ho tlosa matla a phahametseng tlhaho

③.Eketsa 0.6ml ea mokelikeli oa CY1 le 10ul ea proteinase k ho eona, kopanya hantle, 'me ue behe ka har'a seqhoqhoane sa moea se nang le likhato tse 65 tsa Celsius 'me u e kenye metsotso e 30.

④ Centrifuge ho 12000rpm bakeng sa 1min, ntša supernatant eohle ho tube e ncha ea centrifuge, eketsa 10ul magnetic beads le 0.25ml CY2, kopanya hantle bakeng sa 12min, e behe holim'a sethala sa makenete 'me u se tlohele hore se eme bakeng sa 30s ho monya metsi.

⑤ Eketsa 0.6ml ea mokelikeli oa CY3, kopanya hantle bakeng sa 3min, e behe holim'a sethala sa makenete 'me u se tlohele hore se eme 30s ho monya mokelikeli.

⑥.Kenya 0.6ml ea mokelikeli oa CY4, kopanya 3min, e behe holim'a sethala sa makenete 'me u e tlohelle hore e eme 30s ho monya mokelikeli.

⑦.Pheta mohato ⑥

⑧.E omella ka mocheso oa kamore bakeng sa metsotso ea 10-20, eketsa 50ul ea CY5 mokelikeli bakeng sa elution, kopanya hantle, e behe holim'a sethala sa makenete 'me u se lumelle ho ema bakeng sa 30s, ebe u fetisetsa mokelikeli ho tube e ncha ea centrifuge.

⑨.Lekanya OD

Tlhokomeliso: Haeba u hloka ho tlosa RNA, u ka lokisetsa RNaseA 10mg/ml: solvent (10mM sodium acetate: pH5.0), e belise bakeng sa 15min, fetola pH 7.5 ka Tris-Hcl, 'me u boloke ho -20 degrees Celsius.

Maemo a polokelo le nako ea ho sebetsa

1. Sehlahisoa se lokela ho sebelisoa tikolohong e hloekileng le e hloekileng, e qobang tšilafalo, e nang le mocheso o loketseng.

2. Boloka mocheso oa kamore.Proteinase K le lifaha tsa makenete li ka bolokoa ho 2-8°C nako e telele.

3. Bophelo ba sethala sa lihlahisoa: Likhoeli tsa 12

Mehato ya tlhokomelo:

1. Sehlahisoa sena se sebelisoa feela bakeng sa tlhahlobo ea in vitro.

2. Tikoloho ea polokelo le mehato ea ho ntša li lokela ho latela ka tieo litaelo tse bukeng ea litaelo.

3. Haeba u fumana hore chelete e nyenyane haholo nakong ea ho ntša, u ka eketsa boholo ba sampuli ka nepo kapa ua eketsa palo ea lisebelisoa.

4. DNA e nkiloeng e tlameha ho ba e ncha le ho lekoa ka nako.

Tlhokomeliso: Mokhoa ona oa lipalangoang o sebelisetsoa tlhahlobo ea in vitro, 'me o ke ke oa sebelisoa bakeng sa ts'ebeliso ea kahare kapa kantle ho batho kapa liphoofolo.Haeba e metsoa, ​​e ka baka liketsahalo tse tebileng;e hlaba mahlo le letlalo.Haeba ka phoso e phatloha ka mahlong, e hlatsoe ka metsi.E lokela ho kenngoa moea nakong ea tšebeliso.

Selelekela sa moetsi

Huachenyang (Shenzhen) Technology Co., Ltd. e sebetsa ka ho khetheha ho hlahiseng li-swabs tse phallang, li-swabs tsa 'metso, li-swabs tsa molomo, li-nasal swabs, li-cervical swabs, li-sponge swabs, li-tubes tsa sampole ea kokoana-hloko, tharollo ea ho boloka kokoana-hloko.E na le matla a itseng indastering.molemo

Re na le Lilemo tse Fetang 12+ tsa Boiphihlelo ba Tlhahiso ho Lintho tse ka sebelisoang tsa bongaka

HCY e nka boleng ba sehlahisoa e le ntho ea bohlokoa bakeng sa nts'etsopele ea khoebo, ho latela mosuoe-hlooho oa "lihlahisoa tsa boemo ba pele, lits'ebeletso tsa boemo ba pele" ka mokhoa o pota-potileng, ho phehella moea oa khoebo oa "ho batla 'nete, boqapi, bonngoe le katleho" .HCY e hlophisa ts'ebetso eohle ea tlhahiso le thekiso ka mokhoa o tiileng oa tsamaiso ea ISO9001 le ISO13485, ka ts'ebetso e tsitsitseng le boleng bo tšepahalang.


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